Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kdm1a

Cell type

Cell type Class
Neural
Cell type
Cortical neuron
NA
NA

Attributes by original data submitter

Sample

source_name
mouse primary cortical neuron
antibody
Anti- LSD1 Abcam (Cat. No. ab17721)
passages
wild type DIV10
cell type
mouse primary cortical neuron
treatment
KCl
time
1 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated nuclei were precleared with Protein G magnetic beads and antibody chromatin complexes were isolated, and eluted DNA was reverse cross-linked overnight and DNA was purified using Qiagen DNA purification columns using Manufacturer's recommended protocol. Libraries were prepared using Illumina's recommended protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
32805538
Reads aligned (%)
77.7
Duplicates removed (%)
12.6
Number of peaks
745 (qval < 1E-05)

mm9

Number of total reads
32805538
Reads aligned (%)
77.6
Duplicates removed (%)
12.7
Number of peaks
732 (qval < 1E-05)

Base call quality data from DBCLS SRA