Antigen

Antigen Class

TFs and others

Antigen

Kdm1a

Cell type

Cell type Class

Neural

Cell type

Cortical neuron

NA

NA

Sample

source_name

mouse primary cortical neuron

antibody

Anti- LSD1 Abcam (Cat. No. ab17721)

passages

wild type DIV10

cell type

mouse primary cortical neuron

treatment

KCl

time

1 hours

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Sonicated nuclei were precleared with Protein G magnetic beads and antibody chromatin complexes were isolated, and eluted DNA was reverse cross-linked overnight and DNA was purified using Qiagen DNA purification columns using Manufacturer's recommended protocol. Libraries were prepared using Illumina's recommended protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

32805538

Reads aligned (%)

77.7

Duplicates removed (%)

12.6

Number of peaks

745 (qval < 1E-05)

mm9

Number of total reads

32805538

Reads aligned (%)

77.6

Duplicates removed (%)

12.7

Number of peaks

732 (qval < 1E-05)